Composite

Part:BBa_K1197001:Design

Designed by: Alişan Kayabölen   Group: iGEM13_METU_TURKEY   (2013-09-20)


Anti MazF construct (pHyperspank + Anti MazF + Double Terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anti MazF is designed as a complemantary sequence to the last 4 bases of the RBS sequence, 6 bases to the intermediate region and to the first 40 bases of MazF coding sequence, totally 50 bases.

Source

It is synthesized as a reverse complementary sequence to MazF (K302033)


References